Composite

Part:BBa_K2150112

Designed by: Zepeng Mu   Group: iGEM16_UCAS   (2016-10-13)

toxin N-acetyltransferase GCN5 induced by aTc

Usage and Biology

This part is used to build a kill-switch.

Characterization

Growth Curve

We measured the growth curve of bacteria expressing toxin N-acetyltransferase GCN5.

T--UCAS--1204t-atc.png

The growth curve of wild type E. coli harboring empty vector is shown below.

T--UCAS--RGP Ptet.png

Wee also studied the interaction between toxin and antitoxin. See: BBa_K2150116

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 240
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 486
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 240
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 240
    Illegal NgoMIV site found at 417
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None